This hands-on laboratory course provides comprehensive training in experimental techniques central to regenerative medicine and stem cell research. Students will gain practical experience in the culture, maintenance, and characterization of mammalian cells, including pluripotent and adult stem cells, under sterile conditions. The course covers key methodologies such as stem cell expansion, differentiation assays, viability and proliferation analysis, and phenotypic characterization using immunostaining, flow cytometry, and molecular assays.
Students will also be introduced to advanced techniques including organoid generation, 3D culture systems, and basic genome editing approaches using CRISPR-based methods. Practical sessions emphasize experimental design, optimization of culture conditions, troubleshooting, and reproducibility. In addition, students will learn proper laboratory documentation, data recording, and interpretation of experimental outcomes.